The mutant G138P was obtained by in vitro site directed mutagenesis of GI gene.

用双引物法对GI基因进行体外定点突变，构建了GI突变体g 138p。

Human brain derived neurotrophic factor gene was used as template for oligonucleotide based site directed mutagenesis and PCR.

应用寡核苷酸诱导的定点突变和PCR技术对人脑源性神经营养因子基因进行突变，并完成了测序鉴定。

AIM: To establish the mutant of coding calcium binding fragment of the 13th exon of human thrombospondin-1 (TSP-1) gene with polymerase chain reaction (PCR) site directed mutagenesis technology.

目的：利用聚合酶链反应定点突变技术构建人血小板反应素1基因第13外显子编码钙结合域突变体。

Objective: To develop a simple and efficient way to perform site-directed mutagenesis.

目的：介绍一种简便、有效的定点突变技术。

Site-directed mutagenesis is one of the key methods used to study gene function.

定点突变是研究基因功能的一种关键方法。

The reliability of the method was confirmed by site-directed mutagenesis, selective chemical modification, site-specific antipeptide antibody binding experiments, and so on.

大量定点突变实验、选择性化学修饰实验、抗多肽抗体结合实验等能很好地确证该联配结果的正确性。

To clarify the regulatory mechanism, site-directed mutagenesis and reporter gene assay in the CHO cell line were used.

为了阐明调控机制，我们采用了点突变实验和在CHO细胞系中的报告基因分析。

The principles, operation and applications of site-directed mutagenesis, gene fusion technology, and post-translational modification methods were introduced emphatically.

着重阐述了基因定点突变技术、基因融合技术和翻译修饰技术等新兴定点固定化技术的原理、特点和操作。

In the past, the Affinity Labeling and Site-Directed Mutagenesis are the primary methods to study amine receptor.

过去主要是通过亲和标记和点突变等实验方法进行研究。

Site-directed mutagenesis and single-channel patch-clamp recording were used to explore the molecular transitions that underlie inactivation in Shaker potassium channels expressed in Xenopus oocytes.

定点基因突变以及单通道夹被用于探测爪蟾卵细胞中摇动钾离子通道被去活化时的分子转移过程。

Methods The BO mutants I402G and C457S were obtained by site-directed mutagenesis and confirmed by amino acid sequence analysis.

方法胆红素氧化酶突变体i 402g和C457S通过聚合酶链反应获得，并经氨基酸序列测定加以证实。

Methods The polyadenylation signal-deficient retrovirus vector mutated by PCR site-directed mutagenesis was used to make polyadenylation signal-deficient retroviruses by PA317 packaging cells.

方法使用人工定点突变多聚腺苷酸化信号的小鼠逆转录病毒载体，应用PA317病毒包装细胞获得多聚腺苷酸信号缺陷的重组逆转录病毒；

The results indicated that some site-directed mutagenesis can reveal important functional domains of enzyme activity.

结果表明某些定点突变可以揭示影响酶活的重要功能域。