Differential DNA dot hybridization of subtractive cDNA clones.

差减阳性克隆的斑点杂交。

Aim: Cloning and sequencing of hOP-1 cDNA encoding mature protein.

目的：克隆成骨蛋白-1（OP-1）成熟蛋白编码区基因。

Aim: To clone MAR-binding protein cDNA fragment from Dunaliella salina.

目的：克隆杜氏盐藻核基质附着区(MAR)结合蛋白片段。

The lengths of the double stranded cDNA fragments were about 500 base pairs.

其双链链长，经凝胶电泳分析，约为500碱基对。

AIM: To clone and analyze the cDNA encoding immunodiagnostic antigen of cysticercosis.

目的：克隆与分析囊虫病诊断用抗原及其编码基因。

Conclusion a eukaryotic system that expressing mutant CD59 cDNA was successfully set up.

结论建立了两个高效表达突变CD 59的真核表达系统，获得阳性克隆细胞株。

Conclusion: cDNA microarray found the tumor specific gene expression spectrum in gliomas.

结论：基因芯片发现了胶质瘤的异常基因表达谱。

Based on sequence homology, it was suggested that the obtained cDNA was a SAM gene in tea plant.

证实所获得的基因序列为茶树SAM合成酶基因。

Objective To clone the cDNA of the preprotein cDNA of human bone morphogenetic protein 12 (hBMP12).

目的克隆人骨形态发生蛋白12前体蛋白的基因。

Conclusion: The cloned sequence is probably MAR binding protein cDNA fragment from Dunaliella salina.

结论：所克隆的序列可能为杜氏盐藻MAR结合蛋白片段。

RACE could be used to clone the full-length cDNA effectively on the basis of obtaining partial sequence.

在获得一定长度片段的序列基础之上利用RACE法可以有效地进行全长的克隆。

Aim: To develop a PCR technique for rapid screening of recombinant plasmid in subtractive library of cDNA.

目的：消减文库构建过程中，用P CR技术快速筛选重组阳性克隆。

The plasmids in cDNA library and in gene pools were extracted and NIH/3T3 cells were transfected respectively.

将文库菌落印迹至尼龙膜，分区培养提取质粒DNA ，建立基因池，并分别转染NIH/ 3T3细胞。

We have isolated a novel immunoglobulin type cell adhesion molecule, DSCAML1, from a fetal brain cDNA library.

在胎脑文库中获得的DSCAML1属于免疫球蛋白超家族类型的细胞粘附分子。

Objective: To clone cDNA of enamel matrix serine proteinase (EMSP1) encoding mature protein from mouse dental germs.

目的：克隆小鼠牙胚组织中釉基质丝氨酸蛋白酶(EMSP1)成熟肽编码区基因。

The differential gene expression induced by overexpressed CT120B was investigated using Atlas cDNA expression array.

表达阵列分析显示过表达CT120B诱导的基因表达谱变化；

Objective: To study the expressions of pro-onc and tumor suppressor genes in gastric cancer by cDNA expression array.

目的：从基因水平了解正常胃和胃癌组织原癌基因和抑癌基因表达的分子机制。

Objective To explore the tumor related molecular mechanism and functional message from the gigantic cDNA array gene data.

目的探讨从巨量的微阵列基因数据中挖掘肿瘤相关分子机理及功能信息；

Objective: Cloning CD4 cDNA of Homo sapiens to study the mechanism of virus infecting the CD4 + cell for further research.

目的：克隆人的CD 4基因，为进一步研究病毒感染CD 4 +细胞的分子机制奠定基础。

Objective To compare the gene expression status of NB4 cells before and after arsenic sulfide treatment by cDNA microarray.

目的利用基因表达谱芯片对NB 4细胞在硫化砷作用前后基因表达的差异性进行比较。

RESULTS:The results revealed that the sequence of kidney ASBT cDNA gene was identical to that of human intestine ASBT gene.

结果：序列分析结果表明肾小管ASBT基因的序列与小肠ASBT序列完全一致。

Differences in gene expressions were compared by cDNA microarray in skeletal muscle of type 2 diabetic rats and normal rats.

用基因芯片技术比较2型糖尿病大鼠和正常大鼠骨骼肌基因表达谱的差异。

Finally, we could evaluate plasmids cDNA extracted with mono-restriction endonuclease enzyme and the AGAR gel electrophoresis.

最后用限制性内切酶单酶切及琼脂凝胶电泳进行鉴定。

This genetic material can be in the form of a gene, a representative of a gene or cDNA, RNA or even a small fragment of a gene.

这种基因材料可以是基因，基因替代物或cDNA、RNA甚至小的基因片段。

Objective: To clone and sequence a cDNA encoding fibrinolysin metalloproteinase from the venom of Agkistrodon acutus from Guangxi.

目的：克隆桂北五步蛇金属蛋白酶原基因，并对其进行序列分析。

Objective to Screen the various expression of apoptosis related genes between glioblastomas and normal brain tissue with cDNA microarray.

目的研究胶质母细胞瘤凋亡相关基因的表达。

The human novel gene of ZNF322 is cloned from human fetal cDNA library using the primers based on the ZNF322 sequence analyzed with computer.

根据计算机克隆的ZNF322基因序列设计引物，从人类胚胎心脏文库中克隆了ZNF322基因。

A watermelon mosaic virus 2 (WMV-2) isolate from diseased watermelon in Shanxi Province was used for cDNA synthesis of WMV-2 CP gene by RT-PCR.

本研究以从山西西瓜上分离纯化得到的西瓜花叶病毒2号（WMV-2）分离物为材料，用RT-PCR方法得到其外壳蛋白（CP）。

Objective: To construct and identify recombinant adeno-associated virus encoding rat tissue inhibitor of metalloproteinase-1(TIMP-1) full length cDNA.

目的：构建及鉴定载入金属蛋白酶组织抑制因子1（TIMP鄄1）的重组腺相关病毒载体。

Aim To investigate the effect of fibroblast growth factor 2 (FGF2) cDNA mediated by recombinant adeno-associated viral vector2 (rAAV-2) on ischemic myocardium.

探讨重组2型腺相关病毒载体介导2型成纤维细胞生长因子基因诱导家兔缺血心肌血管生成的作用。